ABSTRACT
<p><b>OBJECTIVE</b>To found the subcellular location of the human gene 6 transactivated by nonstructural protein 5A of hepatitis C virus (NS5ATP6).</p><p><b>METHODS</b>Green fluorescent protein (GFP) expression vector pEGFP- NS5ATP6 was established. The pEGFP- NS5ATP6 was transfected into HepG2 cells, and analyze the subcellular location of the proteins expressed by NS5ATP6 through Green fluorescent microscopy after 24 hours.</p><p><b>RESULTS</b>The pEGFP- NS5ATP6 gene was successful cloned, NS5ATP6 can express protein in cells and subcellularly located in cell plasma.</p><p><b>CONCLUSION</b>NS5ATP6 can express protein, and the protein expressed by NS5ATP6 subcellularly located in cell plasma.</p>
Subject(s)
Humans , Cell Line, Tumor , Genetic Vectors , Genetics , Metabolism , Hepacivirus , Intracellular Space , Metabolism , Microscopy, Fluorescence , Transcriptional Activation , Transfection , Viral Nonstructural Proteins , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the clinical therapeutic effects and safety of Fufang Biejia Ruangan tablet (FBRt) in patients with chronic hepatitis B complicated with hepatic fibrosis.</p><p><b>METHODS</b>Totally 420 patients were randomly divided into two groups, FBRt group (300 cases) were treated with Fufang Biejia Ruangan tablets and control group (120 cases) were treated with He Luo Shu Gan capsule, the patients in both groups were treated for 6 months.</p><p><b>RESULTS</b>The cure rate and total effective rate of FBRt group were significantly higher than those of control group (55.67 percent and 81.67 percent vs. 15.8 percent and 60.00 percent, P less than 0.01).</p><p><b>CONCLUSION</b>Fufang Biejia Ruangan tablet could alleviate clinical symptoms and hepatic fibrosis. Fufang Biejia Ruangan tablet is effective and safe in treatment of patients with chronic hepatitis B complicated with liver fibrosis.</p>
Subject(s)
Adolescent , Adult , Aged , Humans , Middle Aged , Alanine Transaminase , Blood , Hepatitis B, Chronic , Drug Therapy , Pathology , Liver , Pathology , Liver Cirrhosis , Drug Therapy , Pathology , Medicine, Chinese Traditional , TabletsABSTRACT
<p><b>OBJECTIVE</b>To study the related factors of the X-ray outcomes in recovered SARS patients.</p><p><b>METHODS</b>The X-ray results of 93 patients with SARS were studied retrospectively. The possible related factors analyzed were age, sex, body temperature at onset, range of the lesion, glucocorticoid administration time. The data were analyzed by chi square test.</p><p><b>RESULTS</b>Among all the patients with abnormal X-ray result, 19 were male (54.29%), 16 (45.71%) were female, P > 0.01; 7 (58.33%) were above the age of 45; 28 (34.57%) were below the age of 45, P > 0.01; hyperpyrexia (>/= 39), 26 (50.00%), below 39, 9 (21.95%); multiple-lesion, 22 (52.38%), mono-lesion, 13 (25.49%), P < 0.01; glucocorticoid administration time within 5 days, 22 (38.60%) after 5 days, 12 (33.33%), P > 0.01; within 7 days, 21 (30.00%), after 7 days, 14 (60.87%), P < 0.01.</p><p><b>CONCLUSION</b>The X-ray results of SARS were closely related to the severity of the disease (hyperpyrexia and bilateral lung field lesion). There was no significant correlation between X-ray result and the age or sex of the patients. Early use of glucocorticoid (within 5 days after onset), had no remarkable influence on the X-ray result. It was noted, however, the incidence of residual lesion in lung obviously increased if glucocorticoid was administered after 7 days of onset.</p>